Abstract
The measurement of intracellular ion concentrations, and the screening of chemical agents to identify molecules targeting ion transport, has traditionally involved low-throughput techniques. Here we present a novel HPLC method that allows the rapid, high-sensitivity measurement of cell Na+ and K+ content, demonstrating its utility by monitoring the ionic changes induced in the intracellular malaria parasite by the new spiroindolone antimalarial KAE609.
Highlights
The measurement of intracellular ion concentrations, and the screening of chemical agents to identify molecules targeting ion transport, has traditionally involved low-throughput techniques
We present a novel HPLC method that allows the rapid, high-sensitivity measurement of cell Na+ and K+ content, demonstrating its utility by monitoring the ionic changes induced in the intracellular malaria parasite by the new spiroindolone antimalarial KAE609
We describe a simple, rapid, high-sensitivity method that allows the quantitation of cell Na+ and K+ from a single trace derived from a single low volume (
Summary
The measurement of intracellular ion concentrations, and the screening of chemical agents to identify molecules targeting ion transport, has traditionally involved low-throughput techniques. We present a novel HPLC method that allows the rapid, high-sensitivity measurement of cell Na+ and K+ content, demonstrating its utility by monitoring the ionic changes induced in the intracellular malaria parasite by the new spiroindolone antimalarial KAE609.
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