Abstract
The design and construction of a computer-controlled Raman spectrophotometer for the determination of difference spectra among up to four samples is described. The advantages of control by a minicomputer compared to a microcomputer or a hard wired controller are discussed. Conditions for the attainment of good signal/noise ratios with aqueous solutions of nucleotides in the 1 to 25 m M range are examined for the case of excitation in the visible spectrum to minimize radiation damage. Applications to studies of dilute aqueous solutions of biological molecules are discussed. The interactions of small molecules with bio-polymers, particularly, are examined.
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