Abstract
Extended abstract of a paper presented at Microscopy and Microanalysis 2011 in Nashville, Tennessee, USA, August 7–August 11, 2011.
Highlights
The recruitment of a fluorescent protein (FP) tagged endocytic protein was referenced to the disappearance of spot-like clathrin coated structure (CCS), but the precision of spot-like CCS disappearance as a marker for canonical clathrin mediated endocytosis (CME) remained unknown
We found scission events engulfed comparable amounts of transferrin receptor cargo at CCS of different sizes and CCS did not always disappear following scission
These data reveal the fine grained temporal structure of clathrin mediated endocytosis (CME) and suggest a simplified canonical model of mammalian CME in which the same
Summary
Dual color total internal reflection fluorescence microscopy (TIR-FM) is a powerful tool for decoding the molecular dynamics of clathrin mediated endocytosis (CME). The recruitment of a fluorescent protein (FP) tagged endocytic protein was referenced to the disappearance of spot-like clathrin coated structure (CCS), but the precision of spot-like CCS disappearance as a marker for canonical CME remained unknown.
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