Abstract
A new method has been devised for the purification and analysis of ecdysones of insect origin. The system uses high-performance liquid chromatography for both purification and analysis, thus allowing a rapid determination of both α- and β-ecdysone in extracts of whole insects. Ecdysone levels analysed during the 12 days of pupal-adult development of Heliothis zea showed a sharp rise in α-ecdysone production one day after larval-pupal ecdysis and a peak concentration 3.5 days after ecdysis. A parallel increase in the titer of β-ecdysone occurred approximately 24 hr later. The concentrations of α and β-ecdysone quickly dropped to low levels on days 5 and 6, respectively, and neither ecdysone was detectable during the latter phase of adult development.
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