Abstract
Abstract A chiral high performance liquid chromatographic method was developed for the simultaneous assay of S(+) and R(−) albuterol in human serum. The assay utilizes solid-phase extraction on a silica column as a sample clean-up step. The chiral separation was accomplished under isocratic conditions using a Sumichiral OA 4700 column and a mobile phase consisting of 350:410:40:2 v/v/v/v hexane/methylene chloride/absolute methanol/trifluoroacetic acid at a flow rate of 1.0 mL/min. The enantiomers were measured using fluorescence detection set at 228 nm excitation and an emission filter of >280nm. Racemic atenolol was used as internal standard. Drug to internal standard peak height ratios were linear over a 2–20 ng/mL range for each enantiomer. The limit of detection of each analyte was 2.0 ng/mL (S/N = 3). The lowest quantifiable level of each enantiomer was 3 ng/mL.
Published Version
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