Abstract
BackgroundThe primary cause of cancer is gene mutation which allows the growth of abnormal and damaged cells. Nutrition is one of the key factors that either increases or decreases the risk of cancer. Mannose has been found in many fruits such as oranges, apples and berries. Mannose has been linked to increase the risk factors or potential therapeutic for cancers. However, insufficient information is available on the effects of high mannose concentration on the normal and cancer cell lines. This study aimed to evaluate the viability patterns of human cancer and normal cell lines treated with mannose. Human gingival fibroblast (HGF), skin malignant melanoma (A375) and colorectal adenocarcinoma (HT29) cell lines were cultured and treated with additional mannose in three respective concentrations: 1 mg/ml, 5 mg/ml and 10 mg/ml. Then, cell viability was measured using an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide)-assay.ResultsThe HGF cells’ percentage pattern of viability showed a rapid decline of nearly 95% on the third day of treatment. A375 cells were able to survive in high mannose condition as the cell viability percentage was at the highest value on Day 5. Meanwhile, HT29 cells showed declining cell viability pattern when treated with mannose. The data exhibited significance; the p value was less than 0.001.ConclusionsHigh mannose concentration can be toxic to HGF. In addition, A375 is adaptive to mannose at all concentrations in which it shares the same pattern with the untreated group. However, the cell viability pattern for HT29 cell is declining.
Highlights
The primary cause of cancer is gene mutation which allows the growth of abnormal and damaged cells
Skin malignant melanoma (A375) is adaptive to mannose at all concentrations in which it shares the same pattern with the untreated group
This study was conducted to evaluate the role of mannose on the viability pattern, the cell lines (human gingival fibroblast (HGF), skin malignant melanoma (A375) and colorectal adenocarcinoma (HT29)) that were treated with mannose at three different concentrations (1 mg/ml, 5 mg/ml and 10 mg/ml)
Summary
The primary cause of cancer is gene mutation which allows the growth of abnormal and damaged cells. Mannose has been linked to increase the risk factors or potential therapeutic for cancers. This study aimed to evaluate the viability patterns of human cancer and normal cell lines treated with mannose. The prime aetiology of cancer is gene mutation as it would lead to the production of abnormal or damaged cells. Necrosis is a mode of cell death that results from the loss of integrity of the plasma membrane and the breakdown of organelles with the release of proteolytic enzymes which subsequently initiate the destruction of the surrounding cells and promote the inflammation [5]. In terms of cell death mechanism, apoptosis is better than necrosis as it avoids the trauma towards the neighbouring cells while necrosis increases the inflammation. Food that could contribute to inflammation need to be controlled
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
