Abstract
Gibberellins (GAs) are a group of phytohormones that have profound and diverse effects on plant growth and development. Within this group, bioactive GAs, including GA1, GA3, GA4, and GA7, play a particularly significant role in regulating these processes. Lychee (Litchi chinensis Sonn.) is a tropical fruit tree native to southern China. Optimization of rapid and precise methods for analyzing bioactive GAs in lychee is crucial to enhance our understanding of the underlying physiological mechanisms of fruit and flower development and to explore its potential industrial applications in this fruit tree. In this study, we developed a high-efficiency method for simultaneous quantitation of bioactive GAs in lychee using ultra-high performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry (UHPLC-QQQ-MS/MS). Ultimately, this method was effectively utilized to quantify the bioactive GAs in various tissues of lychee, including fruits, seeds, and flowers. The developed method exhibited satisfactory recoveries and precision, as well as short chromatographic run time and low limits of detection and quantitation of bioactive GAs in lychee. This protocol provides a valuable method for the characterization of physiological roles and molecular mechanism of GAs in lychee.
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