A Hetero-Multimeric Chitinase-Containing Plasmodium falciparum and Plasmodium gallinaceum Ookinete-Secreted Protein Complex Involved in Mosquito Midgut Invasion.

Kailash P Patra,Hargobinder Kaur,Joseph M Vinetz,Jacob M Wozniak,David J Gonzalez,Chris J Janse,John R Yates,Surendra Kumar Kolli,Judith Helena Prieto

doi:10.3389/fcimb.2020.615343

Abstract

Malaria parasites are transmitted by Anopheles mosquitoes. During its life cycle in the mosquito vector the Plasmodium ookinete escapes the proteolytic milieu of the post-blood meal midgut by traversing the midgut wall. This process requires penetration of the chitin-containing peritrophic matrix lining the midgut epithelium, which depends in part on ookinete-secreted chitinases. Plasmodium falciparum ookinetes have one chitinase (PfCHT1), whereas ookinetes of the avian-infecting parasite, P. gallinaceum, have two, a long and a short form, PgCHT1 and PgCHT2, respectively. Published data indicates that PgCHT2 forms a high molecular weight (HMW) reduction-sensitive complex; and one binding partner is the ookinete-produced von Willebrand A-domain-containing protein, WARP. Size exclusion chromatography data reported here show that P. gallinaceum PgCHT2 and its ortholog, P. falciparum PfCHT1 are covalently-linked components of a HMW chitinase-containing complex (> 1,300 kDa). Mass spectrometry of ookinete-secreted proteins isolated using a new chitin bead pull-down method identified chitinase-associated proteins in P. falciparum and P. gallinaceum ookinete-conditioned culture media. Mass spectrometry of this complex showed the presence of several micronemal proteins including von Willebrand factor A domain-related protein (WARP), ookinete surface enolase, and secreted ookinete adhesive protein (SOAP). To test the hypothesis that ookinete-produced PfCHT1 can form a high molecular homo-multimer or, alternatively, interacts with P. berghei ookinete-produced proteins to produce an HMW hetero-multimer, we created chimeric P. berghei parasites expressing PfCHT1 to replace PbCHT1, enabling the production of large numbers of PfCHT1-expressing ookinetes. We show that chimeric P. berghei ookinetes express monomeric PfCHT1, but a HMW complex containing PfCHT1 is not present. A better understanding of the chitinase-containing HMW complex may enhance development of next-generation vaccines or drugs that target malaria transmission stages.

Highlights

Plasmodium parasites cause human malaria, one of the deadliest vector-borne diseases, which has medical and economic impacts affecting nearly half of the world’s populations (Delves et al, 2019)
In Vitro Production of Plasmodium gallinaceum, Plasmodium falciparum, and Plasmodium berghei Ookinetes in SerumFree and 5% Serum-Containing Media
We demonstrate that Plasmodium falciparum and P. gallinaceum ookinete secrete a short-form chitinase as a covalently-linked, reduction-sensitive, high molecular weight hetero-multimeric protein complex with highest approximate mass of 1,300 kDa

Summary

Introduction

Plasmodium parasites cause human malaria, one of the deadliest vector-borne diseases, which has medical and economic impacts affecting nearly half of the world’s populations (Delves et al, 2019). Despite continuous control measures to reduce transmission, malaria remains a significant public health threat in most tropical and sub-tropical countries (Mlambo et al, 2008). This parasitic human-mosquito-human journey results in more than 400,000 thousand deaths annually; mostly young children under five years of age living in sub-Saharan Africa (Murray et al, 2012; Viebig et al, 2015; Greenwood, 2017). The mosquito midgut gamete, zygote, and ookinete parasite stages are vulnerable developmental bottlenecks in the parasite life cycle and offer opportunities for the elimination of malaria parasites as targets for novel transmission-blocking vaccines, drugs, or transgenic mosquitoes expressing anti-parasite effector molecules (Sinden, 2015; Delves et al, 2018)

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