A herbal medicine decoction, Sibjotang, increases ANP secretion

Abstract

Sibjotang (SJT), which was first documented in the Shanghanlun, has been prescribed for the remedy of body fluid disorders. The present study was designed to investigate the effect of SJT on Atrial natriuretic peptide (ANP) secretion, atrial dynamics and the possible mechanisms in beating rabbit left atria paced electrically. SJT significantly increased ANP secretion and atrial dynamics such as stroke volume and pulse pressure in a concentration‐dependent manner in perfused atria. EK or DG, components of SJT, also significantly increased ANP secretion and atrial dynamics, whereas, there were no significance on ANP secretion and atrial dynamics by treatment of EP or ZJ, respectively. Interestingly, the SJT‐induced increase of ANP secretion was significantly higher than the sum of each EK‐induced increase and DG‐induced increase of ANP secretion (p<0.01). SJT‐induced increase in ANP secretion was attenuated by GO6976, PKC ¥á/¥â inhibitor. In addition, LY333531, PKC ¥â inhibitor, also decreased SJT‐induced ANP secretion.In conclusion, SJT‐induced ANP secretion was potentiated by combination with 4 components in beating rabbit atria. It provides an evidence for synergy effects of a herbal medicine decoction consisting of two or more components. Also, the data suggest that SJT‐induced ANP secretion was related with the activation of PKC ¥á/¥â signaling in perfused beating rabbit atria.