Abstract
We evaluated the anti-atopic dermatitis (AD) effect of Atofreellage (AF), a herbal formula composed of 10 medicinal plants. AD was induced on the dorsal skin areas of NC/Nga mice (male, seven weeks old) by daily application of 2,4-dinitrochlorobenzene (DNCB) for five weeks. After three weeks of DNCB application, 200 μL of AF (0, 25, 50 or 100 mg/mL) was applied to the skin lesions. Histological findings, blood cell populations, serum levels of immunoglobulin E (IgE), histamine, pro-inflammatory cytokines, and inflammatory signaling in the skin tissue, and T-helper cell type 2 (Th2)-related cytokines in splenocytes were analyzed. Histopathological findings showed AF treatment notably attenuated the thickness of dorsal skin, and eosinophil infiltration. AF treatment (especially 100 mg/mL) also demonstrably ameliorated the blood cell population abnormalities, as the notable elevation of serum concentrations of IgE, histamine, TNF-α, IL-6 and IL-1β were remarkably normalized by AF treatment. Western blot analysis evidenced the apparent normalization of inflammatory signals (ERK, p38 MAP kinase, JNK, and NF-κB) in the skin tissue. Additionally, AF treatment notably attenuated the activation of Th2-dominant cytokines (IL-13, IL-4, and IL-5) in Con A-treated splenocytes in an ex vivo assay. In conclusion, this study provides experimental evidence for the clinical relevance of Atofreellage.
Highlights
Atopic dermatitis (AD), a critical medical issue, is a chronically relapsing inflammatory skin disease
[13,14].To To invent an herbal medicine-derived remedy we surveyed the list of plants traditionally used in clinical practice and conducted an experimental screening the medicinal plants traditionally used in clinical practice and conducted an experimental using screening rat-derived basophilic leukemia cell line
AD, but limited success has been achieved to date, and there remains a great demand children, and is increasing in prevalence [15]
Summary
Atopic dermatitis (AD), a critical medical issue, is a chronically relapsing inflammatory skin disease. The morbidity rate of AD has continuously increased, possibly due to industrialization and urbanization [2] Both infants and children are the main subjects suffering from AD, which occasionally distorts healthy development in both physical and emotional terms [3]. The above therapies have limitations regarding their use in use in treating patients withand. Various herbal plants have been used for patients with with skin dermatitis. [13,14].To To invent an herbal medicine-derived remedy we surveyed the list of plants traditionally used in clinical practice and conducted an experimental screening the medicinal plants traditionally used in clinical practice and conducted an experimental using screening rat-derived basophilic leukemia cell line. We aimed to identify the anti-AD effects Atofreellage of Atofreellage investigate underlying pharmacological mechanisms using a.
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