Abstract
A factor capable of lysing erythrocytes arose in the plasma of various strains of mice when the plasma was incubated at 37°C. The hemolytic activity and the amount of lysophosphatidylcholine (lysoPC) in the plasma reached a maximum at 3 h of incubation. Both the hemolytic activity and the increment portion of lysoPC were recovered from the prealbumin fraction on agarose gel electrophoresis. Serum albumin, an inhibitor of the hemolytic action of lysoPC, inhibited the hemolytic action of the this factor. These and other results indicate that the hemolytic factor is a lipoprotein that contains lysoPC as an active site. The molecular weight of active fraction was distributed from 100000 to 200000. The finally purified fraction contained 65% protein composed of a polypeptide with a molecular weight of 25000. The lipid composition of this fraction was 12.4% lysoPC, 63.9% phosphatidylcholine (PC), 5.4% sphingomyelin, 2.1% triacylglycerols and 16.2% cholesterol. This hemolytic lipoprotein was also found in the prealbumin fraction when electrophoresis of plasma of the lysoPC-administered mice was carried out. However, such lipoprotein was not formed by simple addition of lysoPC to the nonincubated plasma.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
