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Abstract
A fluorescence and colorimetric sensor (AHT) was developed to detect HSO3-/SO32- in a 10 mM aqueous PBS buffer at pH 7.4. AHT exhibits non-fluorescence in the buffer due to the intramolecular charge transfer (ICT). The detection of HSO3⁻/SO32⁻ by probe AHT occurs through nucleophilic addition to its carbon-carbon double bond, which disrupts conjugation and stops the ICT process. This reaction induced a visible color change from violet to colorless and activated fluorescence from "off" to "on" at 485nm (λex = 420nm). The probe exhibited significant selectivity and sensitivity for HSO3⁻/SO32⁻, with a detection limit of 51.5 nM and a rapid response time. Additionally, it effectively detected HSO3⁻/SO3²⁻ in real samples, e.g., sugar, water, wine, and biscuits.
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