Abstract
IT was observed during experiments involving the keeping properties of an individual cod liver oil that the value of E1 %1 cm 328 mI¼ determined on the un-saponifiable fraction spontaneously increased, on keeping, to a maximum after several weeks, but the total absorption determined directly on the oil did not increase. The oil had an initial E1 %1 cm 328 mI¼ value of 0.60 when determined on the unsaponifiable matter, and 0.60, 0.62, 0.63, 0.65 and 0.54 after 1, 4, 5, 8 and 14 weeks respectively, whereas the determination on the original oil of 0.70 gave figures of 0.70, 0.69 and 0.67 after 1, 8 and 14 weeks. With tests performed on the oil itself, both by spectrographic means and colour reaction, spontaneous increase in the apparent vitamin A has been noted by others1 and has been assumed due to extraneous substances such as free fatty acids2,3.
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