Abstract
ABSTRACTModification of the headspace gas chromatography method used for ethanol measurement allowed accurate quantitation of acetone in blood. Enzymatic conversion of 3-hydroxybutyrate to acetoacetate and subsequent conversion of the acetoacetate to acetone provided a specific, reproducible method for total ketone body determination. Analysis of blood obtained from sexual assault victims showed that the mean total ketone body concentration (310.1 μmol/L) for all cases was higher than the mean total for blood from control individuals (213.3 μmol/L). There was no significant difference in the total ketone body concentration between the sexual assault victims with visible signs of injury and those with no visible signs of injury (p>0.5). Consumption of ethanol by both groups of sexual assault victims inhibited production of all constituents of the ketone body pool.
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