A green reaction-based turn-off fluorescence sensor for determination of copper ions: DFT calculations, quenching mechanism, green chemistry metrics, and application in environmental samples.

Abstract

When Cu(II) reacts with ascorbic acid (AA) to form Cu(I), Cu(I) can combine with eosin Y (EY) to form ionic associations, resulting in significant fluorescence quenching of the EY. Based on the turn-off of fluorescence in the chemosensor EY, a green reaction is proposed herein for the detection of Cu(II). The novel detection method for Cu(II) demonstrates simplicity, high sensitivity, and excellent selectivity, rendering it suitable for analyzing environmental samples. A static fluorescence quenching mechanism is validated through the Stern-Volmer relationship, and the thermodynamic parameters of the reaction are explored using a van 't Hoff plot. The reaction mechanism is investigated via fluorescence spectra, absorption spectra, and density-functional theory (DFT) calculations. The probe's green nature is confirmed by applying four green analytical chemistry metrics.