Abstract
Recently there is a great social expectation that scientists should produce more sustainable and environmentally friendly chemical processes. Within this necessity, biocatalysis presents many attractive features because reactions are often performed in water, under mild conditions, the catalyst is biodegradable and can be obtained from renewable raw materials. In this work, we propose a simple, rapid and low-cost method for the preparation and application of an enzymatic extract from turnip root. The protocol described includes (1) the preparation of the enzymatic extract, (2) the procedure for the assessment of the more favorable working parameters (temperature, pH) and (3) the methodology for the application of the extract as the catalyst for biotransformation reactions. We anticipate that the protocol in this research will provide a simple way for obtaining an enzymatic extract which can operate efficiently under mild conditions and can effectively catalyze the biotransformation of simple phenols.
Highlights
There is a higher societal awareness regarding the environmental questions of human activities, and science is no exception [1]
Biocatalysis presents many attractive features because reactions are often performed in water, under mild conditions, the catalyst is biodegradable and can be obtained from renewable raw materials
We propose a simple, rapid and low-cost method for the preparation and application of an enzymatic extract from turnip root
Summary
There is a higher societal awareness regarding the environmental questions of human activities, and science is no exception [1] This increasing expectation that scientists should improve processes to become greener and more sustainable, led to the development of green chemistry. Homogeneous catalysts apply to reactions in the gas phase or mostly in the liquid phase These catalysts have some advantages such as excellent selectivity and single active sites, they suffer from some disadvantages, like poor thermal stability and difficult and high cost catalyst recovery [5]. Since they are in a different phase, heterogeneous catalysts can be separated from a reaction mixture, allowing the effective recovery and recycling of the catalyst, which is quite eco-friendly This type of catalysts is very stable, maintaining the activity even under harsh reaction conditions [6].
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