A GREEN AND FACILE APPROACH FOR ANTIDIABETIC AND ANTI-INFLAMMATORY POTENCY FOR FICUS SUBINCISA FRUIT

Abstract

Ficus subincisa belongs to the Moraceae family comprising approximately 850 species. Many Ficus species have been used ethnopharmacologically for the treatment of many health-promoting effects. With increasing economical and ecological concerns for several chemical processes, green chemistry is providing various kinds of “green” solvents that can be a recruit for the extraction and isolation of numerous alleviative and important phytoconstituents from plants. The present study was undertaken to prepare crude extracts of F. subincisa fruits with different polarities of green solvents (d-limonene, isopropyl alcohol, ethyl lactate, and hydroalcohol) by using a modified magnetic stirrer extraction method and assessing in vitro anti-diabetic, anti-inflammatory activities by the spectrophotometric method. Among all, ethyl lactate and hydroalcohol fraction of F. subincisa have shown the highest α-amylase and α-glucosidase enzyme inhibitory activity with an IC50 value of 166.91±2.73 and 118.73±0.67 µg/mL, respectively, which were comparable with that of acarbose. At a concentration of 1000 µg/mL, the hydroalcohol and ethyl lactate of fruit produced 134.53±1.23 and 114.67±4.23 µg/mL inhibition of HRBC hemolysis and bovine serum albumin, respectively, as compared with standard drug aspirin and sodium diclofenac. However, there has been no report on the anti-inflammatory and antidiabetic activity of F. subincisa fruit. Therefore this study was aimed to evaluate the anti-inflammatory and antidiabetic activity of F. subincisa fruit extracts of different green solvents. Our study validated the traditional claim with pharmacological data of the Ficus genus. Taken together, these findings imply that the F. subincisa could be useful therapeutic agents to attenuate muscle insulin resistance due to diet-induced obesity and its associated inflammation.