Abstract
A sensitive, reliable, semiquantitaive organ culture assay has been developed to detect the presence of Mullerian Inhibiting Substance, both in whole testicular fragments, and in cell-free extracts. This in vitro assay allows regression to occur in a manner which mimics the in vivo regression of the male fetal Mullerian duct. Timed exposure studies demonstrate that the Mullerian duct must be exposed to the testicular fragments for at least 12 hr before regression is initiated. Seventy-two hours are required for the morphological changes of regression to be fully expressed. Mullerian Inhibiting Substance is a compound of considerable importance in sexual differentiation. This assay will allow a more systematic approach to the study of this unique fetal regressor.
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