Abstract

A description of the preparatory procedure of the rat gracilis muscle is given. The muscle consists anatomically of two parts, an anterior and a posterior venter, and is supplied by branches of the femoral vessels. As a hemodynamic isolated preparation the gracilis muscle is shown to be applicable to qualitative microcirculatory studies as well as to quantitative measurements on the arteriolar and capillary level. The data presented have been obtained by videomicroscopy, by indirect videophotometric methods, and also by direct techniques like servo-null micropuncture. The use of the preparation described and the results presented are discussed and compared with different methods applied in microcirculation research.

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