A Golgi-targeted fluorescent probe for imaging H2O2 and releasing H2S during Golgi stress

Abstract

Golgi stress was the major stress response in the Golgi apparatus, which might be induced the disruption of redox balance and relate to a variety of diseases. As an important reactive oxygen species (ROS), hydrogen peroxide (H2O2) played an essential role in the Golgi apparatus, the abnormal concentration of H2O2 might cause oxidative damage. In contrast, hydrogen sulfide (H2S) acted as a protector for the Golgi apparatus to relieve Golgi stress. Combined thiocarbamates as H2S donor group activated by oxidizing phenylboronic acid pinacol ester to hydroxyl by H2O2, a Golgi-targeted fluorescent probe Golnap-H2O2 had been developed based on a reported Golgi-targeted fluorophore. Golnap-H2O2 showed the selectivity to H2O2 and the ability to release H2S in the existence of carbonic anhydrase. Besides, Golnap-H2O2 exhibited the targeting ability to Golgi apparatus and was qualified for imaging H2O2. According to our study results, Golnap-H2O2 could not only be used as a satisfactory imaging tool for detecting H2O2 in Golgi apparatus to explore the complex physiological functions, but also be used as a potential H2S donor for cytoprotection.