Abstract
GoldenBraid is a rapid, modular, and robust cloning system used to assemble and combine genetic elements. Dictyostelium amoebae represent an intriguing synthetic biological chassis with tractable applications in development, chemotaxis, bacteria–host interactions, and allorecognition. We present GoldenBraid as a synthetic biological framework for Dictyostelium, including a library of 250 DNA parts and assemblies and a proof-of-concept strain that illustrates cAMP-chemotaxis with four fluorescent reporters coded by one plasmid.
Highlights
Experimental research of the social amoeba Dictyostelium discoideum is highly significant in basic and translational biology and D. discoideum holds immense value as a model organism because of its unique transition from unicellular growth to multicellular development and the tractability of its growth and developmental cycles [1]
The field lacks technologies for high throughput standardized assembly of genetic elements, and the reliance on standard cloning approaches limits the utility of D. discoideum compared to other microbial models
We provide a starter kit of 99 modular genetic elements and 156 useful transcriptional units that are publicly available through the Dictyostelium Stock Center
Summary
Received January 29, 2020; Revised March 06, 2020; Editorial Decision March 10, 2020; Accepted March 11, 2020
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