Abstract
Sea cucumber body wall contains several naturally occurring bioactive components that possess health-promoting properties. Isostichopus badionotus from Yucatan, Mexico is heavily fished, but little is known about its bioactive constituents. We previously established that I. badionotus meal had potent anti-inflammatory properties in vivo. We have now screened some of its constituents for anti-inflammatory activity in vitro. Glycosaminoglycan and soluble protein preparations reduced 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inflammatory responses in HaCaT cells while an ethanol extract had a limited effect. The primary glycosaminoglycan (fucosylated chondroitin sulfate; FCS) was purified and tested for anti-inflammatory activity in vivo. FCS modulated the expression of critical genes, including NF-ĸB, TNFα, iNOS, and COX-2, and attenuated inflammation and tissue damage caused by TPA in a mouse ear inflammation model. It also mitigated colonic colitis caused in mice by dextran sodium sulfate. FCS from I. badionotus of the Yucatan Peninsula thus had strong anti-inflammatory properties in vivo.
Highlights
Sea cucumbers are used as food supplements or traditional medicines in many parts of the world
To ascertain the general nature of the naturally occurring bioactive components in I. badionotus responsible for this ameliorative action, an initial occurring bioactive components in I. badionotus responsible for this ameliorative action, an initial study study was conducted in which three extracts of desalted and lyophilized sea cucumber meal (an was conducted in which three extracts of desalted and lyophilized sea cucumber meal (an ethanolic ethanolic extract [8], a soluble protein extract [9] and a crude glycosaminoglycan (GAGs) preparation extract [8], a soluble protein extract [9] and a crude glycosaminoglycan (GAGs) preparation [10]) were
fucosylated chondroitin sulfate (FCS) Exerts Anti-Inflammatory Activity In Vivo. Given their potent anti-inflammatory activity against keratinocytes in vitro we evaluated the biological activity of the purified FCS in a mouse ear inflammation model
Summary
Sea cucumbers are used as food supplements or traditional medicines in many parts of the world. Harvested species belong to genera including Apostichopus, Atinopyga, Cucumaria, Holothuria, Isostichopus, Parastichopus and Stichopus These are known to contain many potential therapeutic compounds, such as triterpene glycosides (saponins), fucosylated chondroitin sulfates, fucoidans, glycosaminoglycans, sulfated polysaccharides, sterols, phenolics, cerebrosides, lectins and proteins/peptides [1,2]. It remains unclear exactly which compounds or combinations thereof are required to reliably treat or manage specific diseases. Recent work has concentrated on isolation and characterization of individual constituent bioactive compounds from sea cucumber and assessment of their specific health-modulating properties in vitro and in vivo [1,2,3]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
