Abstract

1. 1. A medium more compatible with grasshopper embryonic tissues than a medium previously used, was described. It has been designed easily to permit further modifications. 2. 2. The ratios of sodium to potassium and of chlorides to organic anions are similar to those ratios found in the fluids of larval insects. 3. 3. Mitotic activity of neuroblast cultures in the new medium is maintained at a usable level for 24–48 hours, a much longer period than previously possible. 4. 4. The supplementation of the medium with the amino compounds cysteine, cystine, methionine, and ethanolamine phosphoric acid in addition to the amount provided by the yolk in the hanging-drop cultures, does not affect the level or duration of mitotic activity in neuroblast cultures.

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