Abstract
In this chapter, the β-glucosidase over producing strain Pencillium piceum and the β-glucosidase characteristics will be introduced. Through several rounds of dimethyl sulfate mutagenesis, the β-glucosidase activity of P. piceum reached 53.12 IU/ml. Two new β-glucosidases, promising bifunctional enzymes for lignocellulosic bioconversion, have been found in the extracellular protein of P. piceum. The two new β-glucosidases played an important role in forming multiple soluble cellulose inducers via high transglycosylation activity and novel enzymatic activity. Further, the two new β-glucosidases showed the strong synergism with different cellulases by removing multiple inhibitors for cellulase. The rational computer-aided strategies were devised to enhance the thermostability of the main β-glucosidase (Cel3A) from Penicillium piceum H16. Pencillium piceum, high-yielding β-glucosidase with high enzymatic activity and good thermostability, may provide a good synergetic effect on Trichoderma reesei for improving cellulose hydrolysis of different substrates.
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