A global study of pesticides in bees: QuEChERS as a sample preparation methodology for their analysis – Critical review and perspective

Abstract

To this day, it remains unknown what the cause of decline of honey bee populations is and how to prevent this phenomenon efficiently. Poisonings with pesticides are assumed to be among the main causes for the decline of the honey bee population. Despite the significant progress observed in analytics over recent years, research aimed at improving methods applied in diagnostics of bee poisoning is still in progress. This is no easy task, since determination of the content of trace amounts (often equal to sublethal doses) of a wide range of compounds with diverse physico-chemical properties in honey bee samples with a complex matrix composition poses a serious challenge to modern analytics. This overview is the first to include a comprehensive critical assessment of analytical methods proposed for quantification of pesticides in honey bees over the last decade. Since the QuEChERS method is currently of great significance to ensuring accurate and reliable results of pesticide quantification in honey bees, the present overview focuses on the major aspects of this method, which will provide a comprehensive reference for scientists. The review focuses on the limitations of methods and on potential future prospects. It also contains information on the detection of pesticides in honey bees between 2010 and 2020 and characterizes the pesticide classes which are most toxic to these insects. This is extremely important, not just in the context of understanding the potential adverse impact of pesticides, manifesting as losses in bee colonies; it is also intended to facilitate decision-making in future research related to this difficult yet very important subject.