Abstract
We report the genome sequence of Planobispora rosea ATCC 53733, a mycelium-forming soil-dweller belonging to one of the lesser studied genera of Actinobacteria and producing the thiopeptide GE2270. The P. rosea genome presents considerable convergence in gene organization and function with other members in the family Streptosporangiaceae, with a significant number (44%) of shared orthologs. Patterns of gene expression in P. rosea cultures during exponential and stationary phase have been analyzed using whole transcriptome shotgun sequencing and by proteome analysis. Among the differentially abundant proteins, those involved in protein metabolism are particularly represented, including the GE2270-insensitive EF-Tu. Two proteins from the pbt cluster, directing GE2270 biosynthesis, slightly increase their abundance values over time. While GE2270 production starts during the exponential phase, most pbt genes, as analyzed by qRT-PCR, are down-regulated. The exception is represented by pbtA, encoding the precursor peptide of the ribosomally synthesized GE2270, whose expression reached the highest level at the entry into stationary phase.
Highlights
The genus Planobispora is among the lesser studied genera of actinomycetes [1]
The genome sequence of P. rosea ATCC 53733 was carried out using a whole-genome shotgun approach, which resulted in 117 contigs embedded in 10 scaffolds for a total size of 8.69 Mbp and an overall GC content of 70%
The four largest P. rosea scaffolds, which account for 97.4% of the genome, show considerable synteny with the single-scaffold genome of Streptosporangium roseum NRRL B-2638 [12]
Summary
Despite the first description in 1968 of the soil isolate Planobispora longispora [2], later classified in the family Streptosporangiaceae [3], only three additional species have since been validly described: Planobispora rosea [4], Planobispora siamensis [5] and Planobispora takensis [6] This contrasts with other genera within the Streptosporangiaceae: for example, as of 2006, Streptosporangium and Microbispora had 13 validly described species each [1]. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The funders provided support in the form of salaries for authors [AT, JC, SD and MS], but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific roles of these authors are articulated in the ‘author contributions’ section
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