A genome-wide expression profile analysis reveals active genes and pathways coping with phosphate starvation in soybean.

Qing Wang,Jiao Wang,Wenkai Du,Yuming Yang,Deyue Yu,Dan Zhang,Hao Cheng

doi:10.1186/s12864-016-2558-9

Qing Wang, Jiao Wang + Show 5 more

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https://doi.org/10.1186/s12864-016-2558-9

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Abstract

BackgroundPhosphorus is one of the most important macronutrients that is required for plant growth and development. However, stress under low-P conditions has become a limiting factor that affects crop yields and qualities. Plants have developed strategies to cope with this, while few genes associated with low-P tolerance have been identified in soybean.ResultsGenome-wide analyses were performed on the roots and leaves of a low-P-tolerant accession and a low-P-sensitive accession which were identified by hydroponic experiments under different P treatments. Through comparative analyses on the differently expressed genes, we explored 42 common genes that were highly correlated to low-P stress. The functional classification of these genes revealed 24 Gene Ontology (GO) terms of biological process including response to oxidation reduction, hormone stimuli, and biotic and abiotic stimuli. Additionally, three common pathways were identified.ConclusionsThese results could not only promote the work on the molecular regulation mechanism under low-P stress in soybean, but also facilitate the cultivation of high-phosphorus-acquisition and high-phosphorus-utilization soybean varieties.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-2558-9) contains supplementary material, which is available to authorized users.

Highlights

Phosphorus is one of the most important macronutrients that is required for plant growth and development
In order to alleviate the effects of phosphorus deficiency on soybean growth, it is important for us to search for P-efficient soybean materials and investigate high-phosphorus-efficient genes
Our study provides candidate loci for functional identification of high-phosphorusefficient genes which may be of great significance for cultivating P-efficient soybean accessions

Summary

Introduction

Phosphorus is one of the most important macronutrients that is required for plant growth and development. As an essential constituent of many important compounds that are required for the development and growth of plants, phosphorus (P) actively participates in photosynthesis, respiration, carbohydrate metabolism, energy transduction and other processes [1]. Soybean growth and yield are limited by varieties of edaphic factors, especially low phosphorus availability in soils [3]. Flowering time is increased and the number of pods could decrease during the fruiting period [5]. Due to these P-mediated phenotypic effects on soybeans, phosphate fertilizers are used to overcome undesirable potential yield outcomes. In order to alleviate the effects of phosphorus deficiency on soybean growth, it is important for us to search for P-efficient soybean materials and investigate high-phosphorus-efficient genes

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