A genome-wide analysis of the small auxin-up RNA (SAUR) gene family in cotton

Jiwen Yu,Jinfa Zhang,Man Wu,Wenfeng Pei,Xinshan Zang,Xingli Li,Honghong Zhai,Yanhui Geng,Guoyuan Liu,Xihua Li,Shuxun Yu

doi:10.1186/s12864-017-4224-2

Abstract

BackgroundSmall auxin-up RNA (SAUR) gene family is the largest family of early auxin response genes in higher plants, which have been implicated in the regulation of multiple biological processes. However, no comprehensive analysis of SAUR genes has been reported in cotton (Gossypium spp.).ResultsIn the study, we identified 145, 97, 214, and 176 SAUR homologous genes in the sequenced genomes of G. raimondii, G. arboreum, G. hirsutum, and G. barbadense, respectively. A phylogenetic analysis revealed that the SAUR genes can be classified into 10 groups. A further analysis of chromosomal locations and gene duplications showed that tandem duplication and segmental duplication events contributed to the expansion of the SAUR gene family in cotton. An exon-intron organization and motif analysis revealed the conservation of SAUR-specific domains, and the auxin responsive elements existed in most of the upstream sequences. The expression levels of 16 GhSAUR genes in response to an exogenous application of IAA were determined by a quantitative RT-PCR analysis. The genome-wide RNA-seq data and qRT-PCR analysis of selected SAUR genes in developing fibers revealed their differential expressions. The physical mapping showed that 20 SAUR genes were co-localized with fiber length quantitative trait locus (QTL) hotspots. Single nucleotide polymorphisms (SNPs) were detected for 12 of these 20 genes between G. hirsutum and G. barbadense, but no SNPs were identified between two backcross inbred lines with differing fiber lengths derived from a cross between the two cultivated tetraploids.ConclusionsThis study provides an important piece of genomic information for the SAUR genes in cotton and lays a solid foundation for elucidating the functions of SAUR genes in auxin signaling pathways to regulate cotton growth.

Highlights

Small auxin-up RNA (SAUR) gene family is the largest family of early auxin response genes in higher plants, which have been implicated in the regulation of multiple biological processes
The putative SAUR members were analyzed for conserved domains using the HMMER and Pfam programs, leading to the identification of 145, 97, 214, and 176 SAUR genes in G. raimondii, G. arboreum, G. hirsutum, and G. barbadense, respectively (Table 1; Additional files 2, 3 and 4: Table S2, S3, S4)
We observed that tandem duplication and segmental duplication events contributed to the expansion of the SAUR gene family in cotton

Summary

Introduction

Small auxin-up RNA (SAUR) gene family is the largest family of early auxin response genes in higher plants, which have been implicated in the regulation of multiple biological processes. Most of early regulated auxin responsive genes are classified into three families: Aux/IAA, Gretchen Hagen (GH3), and small auxin-up RNA (SAUR) [3]. Since the first SAUR gene was identified in elongating soybean hypocotyl sections [5], members of this gene family have been identified by genome-wide analyses in diverse plant species, such as Arabidopsis [3], rice [6], sorghum [7], tomato [8], potato [8], maize [9], citrus [10], and ramie [11]. In Arabidopsis, the overexpression of AtSAUR19 subfamily proteins (AtSAUR19 to AtSAUR24) with a N-terminal tag resulted in root waving, increased hypocotyl elongation and leaf size, defective apical hook maintenance, and

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