Abstract
Powdery mildew is a widespread fungal plant disease that can cause significant losses in many crops. Some MLO genes (Mildew resistance locus O) have proved to confer a durable resistance to powdery mildew in several species. Resistance granted by the MLO gene family members has prompted an increasing interest in characterizing these genes and implementing their use in plant breeding. Lentil (Lens culinaris Medik.) is a widely grown food legume almost exclusively consumed as dry seed with an average world production of 4.5 million tons. Powdery mildew causes severe losses on certain lentil cultivars under particular environmental conditions. Data mining of the lentil CDC Redberry draft genome allowed to identify up to 15 gene sequences with homology to known MLO genes, designated as LcMLOs. Further characterization of these gene sequences and their deduced protein sequences demonstrated conformity with key MLO protein characteristics such as the presence of transmembrane and calmodulin binding domains, as well as that of other conserved motifs. Phylogenetic and other comparative analyses revealed that LcMLO1 and LcMLO3 are the most likely gene orthologs related to powdery mildew response in other species, sharing a high similarity with other known resistance genes of dicot species, such as pea PsMLO1 and Medicago truncatula MtMLO1 and MtMLO3. Sets of primers were designed as tools to PCR amplify the genomic sequences of LcMLO1 and LcMLO3, also to screen lentil germplasm in search of resistance mutants. Primers were used to obtain the complete sequences of these two genes in all of the six wild lentil relatives. Respective to each gene, all Lens sequences shared a high similarity. Likewise, we used these primers to screen a working collection of 58 cultivated and 23 wild lentil accessions in search of length polymorphisms present in these two genes. All these data widen the insights on this gene family and can be useful for breeding programs in lentil and close related species.
Highlights
Powdery mildew is an airborne plant disease widespread in temperate climates that is caused by ascomycete fungi of the order Erysiphales and that can cause significant harvest losses in field-crops, fruit crops, and ornamental plants
Eight sequences predicted to encode truncated proteins were identified. These shorter sequences were considered pseudogenes and named according to the M. truncatula gene designation following the same procedure
Most likely pseudogenes, were found in lentil genome, any of them were identified as orthologs of the MtMLO12 and MtMLO16 psudogenes described in M. truncatula [20]
Summary
Powdery mildew is an airborne plant disease widespread in temperate climates that is caused by ascomycete fungi of the order Erysiphales and that can cause significant harvest losses in field-crops, fruit crops, and ornamental plants. The Mildew resistance locus O (MLO) gene. MLO genes in lentil demonstration under grant agreement n 613551 (LEGumes for the Agriculture of Tomorrow; LEGATO) to Marcelino Perez de la Vega and partially supported by the Spanish Ministerio de Economıa y Competitividad (grant AGL201344714-R to Marcelino Perez de la Vega, cofinanced with FEDER funds) and the Junta de Castilla y Leon to Research Groups (GR113) to Marcelino Perez de la Vega
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