Abstract
AbstractThe genetic control of frost resistance was studied in callus cultures using some of the chromosome substitution lines of the variety ‘Cheyenne’ into ‘Chinese Spring’. The survival of the calli derived from immature embryos was studied with triphenyltet‐razolium chloride (TTC) and fluorescein diacetate (5DA) methods after hardening and freezing at a temperatures of ‐7 °C, ‐9 °C, ‐11 °C, ‐13 °C, and –15 °C. The donor ‘Cheyenne’ and the substitution lines 5A and 5D proved to be more frost resistant than the recipient ‘Chinese Spring1. These results are well correlated with the previously published studies when seedlings were tested under controlled conditions. Based on these results the tissue culture technique seems to be useful for testing varieties and lines for different levels of frost resistance and even for mutant selection.
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