A GENETIC LINKAGE MAP OF OLEA EUROPAEA L. USING A PSEUDO-TEST CROSS- MAPPING STRATEGY BASED ON SSR, AFLP, ISSR, RAPD AND SCAR MARKERS

Abstract

Abstract A genetic map of olive tree ( Olea europaea ; 2n = 46) was constructed with a total of 343 markers: 35 SSRs, 272 AFLPs, 35 ISSRs, and RAPD and SCAR scored in 140 F 1 progenies from the cross between ‘Picholine marocaine’ ‘PM’ (female parent) and ‘Picholine du Languedoc’‘PL’ (male parent), respectively susceptible and resistant to Spilocaea oleagina. The female map consisted of 100 markers clustered in 24 linkage groups (LG) and covered over 1260.9 cM, while the male map was defined by 90 markers assigned to 22 linkage groups and covered 1183 cM. The consensus map obtained based on 193 markers (26 SSRs, 151 AFLPs, 12 ISSRs, 3 RAPDs, 1 SCAR) and 31 linkage groups covered 2332.8 cM. INTRODUCTION The olive tree ( Olea europaea ; 2n =46 ) is one of the first cultivated Mediterranean fruit species with an important economical value, particularly during the last two decades. In Morocco, ‘PM’ is the dominant cultivar, with more than 98% of the olive orchards (Boulouha et al., 1992). However, this variety is susceptible to the peacock spot disease attributed to the fungus