Abstract
A linkage map of the peach (Prunus persica (L.) Batsch), was constructed by using 133 F2 plants from the intraspecific cross between the rootstock cultivar, 'Akame', and the flowering ornamental peach, 'Juseitou'. This map contains 83 markers, including 3 morphological trait loci, brachytic dwarf (dw), red leaf (Gr) and narrow leaf (nl), 41 RAPD markers, 30 AFLP markers, and Inter-SSR, PCR-RFLP markers. Ten linkage groups were found, ranging from 17 to 244 cM in genetic distance ; among these, the 6 major groups had genetic distances of more than 60 cM. The map covers more than 960 cM with an average density of 12 cM between markers. A newly designated morphological characteristic, narrow leaf character (designated nl) was found to co-segregate with the dw locus. We found that 3 morphological traits, dw (nl) were located on the same linkage group as the Gr locus at a distance of 65 cM. Several DNA markers were linked to Gr and dw loci, which could be utilized not only for selecting morphological traits but also for evaluating homozygosity or heterozygosity. For example, the 95 bp AFLP fragment produced from the E-AGT/M-CAA primer combination, is tightly linked to the Gr locus at a distance of 5.0 cM. The DNA markers obtained are horticulturally very important and will be utilized for peach breeding.
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