Abstract
THE cytolytic effect of immune serum has been the subject of study by a number of investigators. It has been shown1 that cells other than erythrocytes undergo lytic changes in the presence of antibody and complement. It has been further shown2, using cells labelled with phosphorus-32, that the lysis of such cells is accompanied by a release of intracellular proteins, similar to the release of haemoglobin from lysed erythrocytes. It appeared that the immune lysis of tissue cells is quite analogous to the immune lysis of erythrocytes. On this basis, a test was developed using radioactive labelled cells which provides a means of quantitating immune lysis for cells derived from a variety of tissues.
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