A gas-driven capillary based on the synergy of the catalytic and photothermal effect of PB@Au for Salmonella typhimurium detection

Abstract

Rapid detection method for Salmonella typhimurium is vital to prevent the spread of food-borne diseases. In this work, a gas-driven capillary detection method was established to achieve sensitive and rapid detection of Salmonella typhimurium using the catalytic and photothermal synergy of Prussian blue-nanogold (PB@Au) nanomaterials. The immuno-PB@Au probe attached to the capillary by specific identification of target bacteria catalyzed the H2O2 under laser irradiation, driving the H2O2 liquid column to move (ΔL) by producing gas, and achieving the quantitative detection of Salmonella typhimurium. After detailed optimization of the critical performance parameters of the gas-driven capillary assay, the limit of detection (LOD) after laser irradiation and being catalyzed by PB@Au was calculated to be 37 CFU mL−1 through the determination of different concentrations of target bacteria. Furthermore, the detection performances of the gas-driven capillary method were evaluated in detail, and the recoveries ranging from 92.9 ± 4.7 % to 107.7 ± 4.1 % were achieved using the spiked actual samples with complex matrices, indicating that the established rapid assay can offer promising strategies for the monitoring and controlling of food-borne pathogenic bacteria.