Abstract
Penicillic acid was satisfactorily resolved by gas chromatography either as the trimethylsilyl derivative or in the underivatized form using 3% of Dexsil 300, 3% of OV-17 or 3% of OV-25 liquid phase on Gas-Chrom Q. The limit of quantitation was approximately 25 ng. Extraction and detection of penicillic acid together with another important mycotoxin, patulin, was demonstrated in moldy corn. In addition; a preliminary clean-up procedure using thin-layer chromatography is described for crude extracts, which improves resolution and sensitivity in the gas chromatographic analysis of penicillic acid.
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