Abstract
In the course of a routine paternity test for White Shepherd dogs using microsatellite T2201 an apparent non‐Mendelian inheritance was seen. It could be shown that the observed phenomenon was caused by a G > C transversion within the reverse primer binding site of one maternal allele leading itself to a ‘null’ allele. Using an alternative primer complementing for the transversion the ‘null’ allele and all other known alleles of the microsatellite T2201 in White Shepherd dogs can be amplified under standard polymerase chain reaction conditions. Although the frequency of this ‘null’ is very low (0.08) we recommend the use of the alternative primer for paternity testing and related applications (e.g. genotype data bank) in this breed.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
