Abstract
Real-time and accurate detection of endogenous hydrogen sulfide is of great biomedical significance. Here, a FRET-based fluorescent probe for ratiometric detection of H2S was designed to comprise an AIE luminophore TPE as an energy donor and a monochlorinated BODIPY dye as an energy acceptor and H2S-responsive site. Such a designed probe showed H2S-dependent ratiometric and light-up NIR-II emission, enabling accurate imaging of H2S-rich cancer cells and identification of H2S-rich tumors with high resolution.
Highlights
Endogenous hydrogen sulfide is an important signaling molecule, mainly derived from the enzymatic hydrolysis of L-cysteine (Chiku et al, 2009; Singh et al, 2009)
Real-time and accurate detection of hydrogen sulfide is of great biomedical significance
We reported a H2S-responsive probe that showed ratiometric fluorescence and NIR-II emission light-up upon activation for in vitro and in vivo imaging (Scheme 1). Such a probe was designed by appending an AIE luminophore TPE, as an energy donor, to a monochlorinated BODIPY dye as an energy acceptor and H2S-responsive site
Summary
Endogenous hydrogen sulfide is an important signaling molecule, mainly derived from the enzymatic hydrolysis of L-cysteine (Chiku et al, 2009; Singh et al, 2009). Compared with the traditional NIR-I imaging (650–900 nm) (Li et al, 2018), fluorescent imaging in the second near-infrared window (NIR-II, 1,000–1,700 nm) has attracted more and more attention due to lower tissue absorption, stronger tissue penetration, and reduced autofluorescence (Hong et al, 2012; Dang et al, 2016; Shi et al, 2018; Xu et al, 2018) Another issue is the hydrophobic nature of most traditional fluorescent dyes, which generally triggers the aggregation in physiological conditions due to π-π stacking. We reported a H2S-responsive probe that showed ratiometric fluorescence and NIR-II emission light-up upon activation for in vitro and in vivo imaging (Scheme 1) Such a probe was designed by appending an AIE luminophore TPE, as an energy donor, to a monochlorinated BODIPY dye as an energy acceptor and H2S-responsive site. HRMS (ESI, m/z): calculated for C58H50BF2N3Cl [M-Br]+: 872.3754, found: 872.3750
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