A FRET-based ratiometric fluorescent probe for SO32− detection in Chinese medicine and living cells

Abstract

Chinese herbal medicine is receiving more and more attention at home and abroad as a traditional Chinese clinical medicine. To make herbal medicines can be preserved for a long time, they are usually fumigated with sulfur. However, after the medicinal materials have been fumigated with sulfur, SO2 residues will remain, which, when exposed to water, will create sulfites and bisulfites. Excessive sulfites can cause a variety of severe ailments and diminish the quality and effectiveness of therapeutic plants. Therefore, developing an effective SO32−/HSO3− detection method is important. This study chose coumarin derivatives as fluorescent acceptors and pyridinium acrylonitrile structures as fluorescent donors to create a ratiometric fluorescent probe CPA using the fluorescence resonance energy transfer (FRET) effect. The probe CPA exhibited a fluorescence transition from red to green under excitation at 405 nm with an interval of 149 nm, a reaction time of less than 1 min, a low detection limit of 86 nM, and the probe CPA has good specific recognition of SO32− and is resistant to interference. In addition, CPA has low in vitro cytotoxicity and can successfully detect endogenous sulfites in living cells.