Abstract
Tailored supercritical carbon dioxide (scCO 2) extraction of alkylresorcinols (ARs) from rye bran resulting in pre-purification of ARs already during extraction process. The pre-purification was achieved by splitting the extraction process in 2 steps. . In both steps, the CO 2 pressure was set at 25 MPa with a flow rate of 10 g/min. The first step was carried out at 70 °C, 0.06% of ethanolic co-solvent for 2 h, followed by the second step at 45 °C using 10% of ethanolic co-solvent for 4 h, resulting in a pre-purified ARs extract. From the pre-purified scCO 2 extract, chromatographed on C8 column, were obtained pure ARs homologues of C17, C19 and C21 (68% of total) eluting separately in a linear gradient of ethanol. Additionally, a rapid method for the isolation of high purified pools of ARs homologues using a disposable solid phase extraction SPE-C18 column and a step gradient of ethanol was developed.
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