A Follow-Up to the Geographical Distribution of Anopheles Species in Malaria-Endemic and Non-Endemic Areas of Honduras

Abstract

Simple SummaryMalaria is a tropical disease caused by parasites of the genus Plasmodium. The parasite is transmitted to humans through the bite of the female mosquito Anopheles. Honduras is close to the goal of eliminating malaria, but the region called La Moskitia continues to concentrate almost all of the country’s malaria cases. One of the key factors in achieving malaria elimination is a thorough understanding of the mosquito vectors that transmit the disease. There are few studies related to malaria vectors in Honduras. This study aims to contribute to knowing which are the species of vector mosquitoes, mainly in the Department of Gracias a Dios and in other departments in which cases of malaria occur, in addition to describing molecularly for the first time the anophelines of the Bay Islands. The most abundant species found here were Anopheles albimanus, but seven other species were also identified, some of which may contribute to parasite transmission.Anopheles species are the vectors of malaria, one of the diseases with the greatest impact on the health of the inhabitants of the tropics. Due to their epidemiological relevance and biological complexity, monitoring of anopheline populations in current and former malaria-endemic areas is critical for malaria risk assessment. Recent efforts have described the anopheline species present in the main malaria foci in Honduras. This study updates and expands knowledge about Anopheles species composition, geographical distribution, and genetic diversity in the continental territory of Honduras as in the Bay Islands. Outdoor insect collections were carried out at 25 sites in eight municipalities in five departments of Honduras between 2018 and 2021. Specimens were identified using taxonomic keys. Partial COI gene sequences were used for molecular species identification and phylogenetic analyses. In addition, detection of Plasmodium DNA was carried out in 255 female mosquitoes. Overall, 288 Anopheles mosquitoes were collected from 8 municipalities. Eight species were morphologically identified. Anopheles albimanus was the most abundant and widely distributed species (79.5%). A subset of 175 partial COI gene sequences from 8 species was obtained. Taxonomic identifications were confirmed via sequence analysis. Anopheles albimanus and An. apicimacula showed the highest haplotype diversity and nucleotide variation, respectively. Phylogenetic clustering was found for An. argyritarsis and An. neomaculipalpus when compared with mosquitoes from other Neotropical countries. Plasmodium DNA was not detected in any of the mosquitoes tested. This report builds upon recent records of the distribution and diversity of Anopheles species in malaria-endemic and non-endemic areas of Honduras. New COI sequences are reported for three anopheline species. This is also the first report of COI sequences of An. albimanus collected on the island of Roatán with apparent gene flow relative to mainland populations.