Abstract
A chemical method for the determination of folic acid in pharmaceutical products is presented. The method involves digesting the folic acid in a mildly alkaline, buffered solution (3% K 2 HPO 4 ) followed by splitting with KMnO 4 , diazotizing the resulting PABA, and coupling with the Bratton-Marshall reagent ( n -1-naphthyl)-ethylene-diamine dihydrochloride. In the presence of extraneous colored substances, as may be found in some pharmaceutical preparations, the chromogenic compound is extracted into isobutyl alcohol with no diminution in intensity of color, or change in wavelength of maximal absorption (550 mμ). Ferrous compounds and ascorbic acid do not interfere in the method as they are found to do in the NH 4 OH digestion-zinc reduction method. The assay time with the proposed method is less because of the shorter time required to cleave folic acid by oxidation. The results are reproducible, and good folic acid recoveries are obtained.
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