Abstract
Grapevine stilbenes are a family of polyphenols which derive from trans-resveratrol having antifungal and antimicrobial properties, thus being considered as phytoalexins. In addition to their diverse bioactive properties in animal models, they highlight a strong potential in human health maintenance and promotion. Due to this relevance, highly-specific qualitative and quantitative methods of analysis are necessary to accurately analyze stilbenes in different matrices derived from grapevine. Here, we developed a rapid, sensitive, and specific analysis method using ultra-high-performance liquid chromatography coupled to triple-quadrupole mass spectrometry (UHPLC-QqQ) in MRM mode to detect and quantify five grapevine stilbenes, trans-resveratrol, trans-piceid, trans-piceatannol, trans-pterostilbene, and trans-ε-viniferin, whose interest in relation to human health is continuously growing. The method was optimized to minimize in-source fragmentation of piceid and to avoid co-elution of cis-piceid and trans-resveratrol, as both are detected with resveratrol transitions. The applicability of the developed method of stilbene analysis was tested successfully in different complex matrices including cellular extracts of Vitis vinifera cell cultures, reaction media of biotransformation assays, and red wine.
Highlights
Grapevine (Vitis vinifera L.) stilbenes are a family of polyphenols which derive from trans-Resveratrol, found in different plant organs, including berries and, in wines
As this instrument uses a fixed 380 V fragmentor voltage this parameter cannot be optimized. Glycosylated polyphenols, such as piceid, are prone to in-source fragmentation, it was necessary to optimize some parameters of the ion source to minimize this effect
In the context of the occurrence of qualitatively bioactive stilbenes in naturalthesources, liquid compounds in different matrices is necessary.toThe way both towards efficient analytical methods ofthe chromatography-based effective methodology analyze qualitatively and quantitatively polyphenols been focused in making both a is more efficientThe chromatographic and a content of thesehas compounds in different matrices necessary
Summary
Grapevine (Vitis vinifera L.) stilbenes are a family of polyphenols which derive from trans-Resveratrol (trans-R: 3,5,40 -trihydroxystilbene), found in different plant organs, including berries and, in wines. BeenMany demonstrated to major human diseases such astocancer, neurodegeneration studies have have a large number of bioactive properties in relation to major human diseases such as cancer, reported the potential of trans-R in human health as an antioxidant in model systems of cardiovascular atherosclerosis, or neurodegeneration [10]. Other studies have demonstrated exhibit enhanced pharmacological activity and bioavailability [21] Such potential of trans-R and that some trans-R polyhydroxy and polymethoxy derivatives exhibit enhanced pharmacological other stilbenes have called the attention of numerous companies producing a large variety of dietary activity and bioavailability [21]. Such potential of trans-R and other stilbenes have called the attention supplements and cosmetics. Cocoa, and peanuts, andsuch theirasderived this sense, especially are presently in natural foods, grapes, products berries of [22,23,24,25,26]
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