Abstract
We screened salivary glands from adult deer ticks collected near Spooner and Hayward, Wisconsin, to determine whether deer tick virus, a recently described flavivirus, occurs with other tickborne agents in the upper Midwest. Intraacinar inclusions suggestive of replicating virus were detected in 4 (4.6%) of 87 ticks. The virus was isolated by suckling-mouse inoculation.
Highlights
We screened salivary glands from adult deer ticks collected near Spooner and Hayward, Wisconsin, to determine whether deer tick virus, a recently described flavivirus, occurs with other tickborne agents in the upper Midwest
In addition to the agents of Lyme disease (Borrelia burgdorferi), human granulocytic ehrlichiosis (Ehrlichia microti), and human babesiosis (Babesia microti), deer ticks in these foci might be infected with deer-tick virus (DTV), a recently described Powassan (POW)-like agent of the genus Flavivirus [5], and might pose an additional threat to human health in this region
The other was pooled with the salivary glands of four other ticks in Hanks’ Balanced Salt Solution supplemented with fetal bovine serum (HBSS/FBS) for mouse inoculation and polymerase chain reaction (PCR) analysis
Summary
We screened salivary glands from adult deer ticks collected near Spooner and Hayward, Wisconsin, to determine whether deer tick virus, a recently described flavivirus, occurs with other tickborne agents in the upper Midwest. In addition to the agents of Lyme disease (Borrelia burgdorferi), human granulocytic ehrlichiosis (Ehrlichia microti), and human babesiosis (Babesia microti), deer ticks in these foci might be infected with deer-tick virus (DTV), a recently described Powassan (POW)-like agent of the genus Flavivirus [5], and might pose an additional threat to human health in this region. To determine whether this newly recognized agent infects deer ticks in a focus outside New England, we sampled ticks from two heavily infested sites in northern Wisconsin and assayed them for all four agents.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
