Abstract
INTRODUCTION THE biological assay for estrogens based on the vaginal response in ovariectomized rats was first standardized by Kahnt and Doisy (1). This method has been used by many investigators for the estimation of estrogenic activity in various biological fluids. However, biological assay has the disadvantage of being long, expensive and subject to variations inherent in animals. It also necessitates the maintenance of a large animal colony. Several investigators have proposed colorimetric chemical methods for estimating the estrogen content of urine in order to avoid biological assay (Kober (2), David (3), Talbot et al. (4), Szego and Samuels (5)). The urine of normal males and females contains such small concentrations of estrogens that the methods proposed usually lack sufficient sensitivity. This necessitates the use of large quantities of urine, so that nonspecific urinary chromogens interfere with accurate determination. During the past several years, fluorometric methods have been perfected to ...
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