Abstract
An electrogenerated chemiluminescence (ECL) based method is described for the determination of hydrogen peroxide (H2O2) released by living cells. It is making use of a fluorine-doped tin oxide (FTO) electrode that was modified with gold nanoparticles (AuNPs). The AuNP-FTO was fabricated by electrodeposition of AuNP on the surface of the FTO electrode and characterized by scanning electron microscopy and electrochemical techniques. The AuNP-FTO exhibits a significant electrocatalytic activity towards luminol-based ECL system compared to a bare FTO. The calibration plot covers the 10 nM to 1 μM H2O2 concentration range, with a 8 nM detection limit (at a signal-to-noise ratio of 3). The method enabled (a) the monitoring of drug-stimulated production of H2O2 released by living cells including RAW 264.7 macrophage cells and HeLa cells; and (b) the determination of inhibitors of H2O2 secretion. The method, due to strong signal amplification by AuNPs, provides a viable tool for determination of H2O2 in biological systems.
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