Abstract
AbstractA rhodamine‐based copper complex as a selective, sensitive turn‐on fluorescent chemosensor for NO/histidine has been developed. A conspicuous fluorescence enhancement was observed in the presence of nitric oxide/histidine. The probe was specific towards NO over other reactive oxygen species and reactive nitrogen species. The probe showed selective fluorescence enhancement with histidine; the other naturally occurring amino acids did not result in fluorescence enhancement. EPR and ESIMS studies clearly showed that NO‐induced reduction of copper ions leads to the fluorescence enhancement. The viability of the probe for fluorescent imaging of nitric oxide and histidine in living cells has been demonstrated by means of confocal laser scanning microscopy experiments.
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