Abstract
In this article, a fluorescent probe FCZ with fluorescein-carbazole as the basic skeleton was designed and synthesized. In contrast to the presences of other coexisting anions, active oxygen and organic thiols, the probe could be "turn-on", exhibiting specific fluorescence performance towards hypochlorite (ClO-). Comprehensive analyses by electrospray ionization mass spectrometry (ESI-MS), thin-layer chromatography (TLC), nuclear magnetic resonance (NMR), and density functional theory/time-dependent density functional theory (DFT/TDDFT) revealed that ClO- could react with the imine bond of the probe, forming fluorescein, resulting in a significant increase of fluorescence emission intensity. The probe has a detection limit for ClO- in water of 0.056 μmol/L. In addition, the probe was successfully applied to detect ClO- in water samples, as well as in the imaging of ClO- in RAW264.7 cells and zebrafish.
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