Abstract
Objective: This study intends to identify potential prognostic marker genes associated with the prognosis of patients suffering from hepatocellular carcinoma (HCC) based on TCGA and GEO analysis.Methods: TCGA-LIHC cohort was downloaded and the data related to HCC were extracted from The Cancer Genome Atlas (TCGA) database and subjected to differential analysis. HCC-related gene expression datasets were retrieved from the GEO database, followed by differential analysis. After intersection of the results of TCGA and GEO databases, gene interaction analysis was performed to obtain the core genes. To identify the genes related to the prognosis of HCC patients, we conducted univariate and multivariate Cox analyses.Results: Based on differential analysis of TCGA database, 854 genes were differentially expressed in HCC, any of which might link to the occurrence and progression of HCC. Meanwhile, joint analysis of HCC-related gene expression datasets in the GEO database screened 214 genes. Five core genes CDC20, TOP2A, RRM2, UBE2C and AOX1 were significantly associated with the prognosis of HCC patients and the risk model based on these five genes effectively predicted the prognosis of HCC patients.Conclusion: Collectively, our data suggest that CDC20, TOP2A, RRM2, UBE2C and AOX1 may be the key genes affecting the prognosis of patients with HCC. The five-gene signature could accurately predict the prognosis of HCC patients.
Highlights
Hepatocellular carcinoma (HCC) is identified as the most frequently occurring primary liver cancer as well as one of the leading reasons for cancer-related death on a global scale [1]
In biological process (BP) category, these differentially expressed genes were mainly enriched in steroid metabolism and fatty acid metabolism (Figure 2A), while in the cellular component (CC) category, these genes were primarily enriched in vesicle related items (Figure 2B)
The analysis revealed a suite of five genes, Cell division cycle 20 (CDC20), TOP2A, ribonucleotide reductase subunit M2 (RRM2), ubiquitin-conjugating enzyme 2C (UBE2C) and alcohol oxidase 1 (AOX1), that may be the key genes affecting the prognosis of HCC
Summary
Hepatocellular carcinoma (HCC) is identified as the most frequently occurring primary liver cancer as well as one of the leading reasons for cancer-related death on a global scale [1]. Screening of Molecular Markers of HCC Prognosis. Cell division cycle 20 (CDC20), in combination with G2 and S-phase expressed 1 (GTSE1), proliferating cell nuclear antigen (PCNA), and minichromosome maintenance complex component 6 (MCM6), could have effects on cell cycle of HCC, while serving as markers for poor prognosis [5]. According to a previous study, topoisomerase II alpha (TOP2A) and ribonucleotide reductase subunit M2 (RRM2) were upregulated hub genes in HCC, and shared an association with lower survival rate of patients with HCC [7]. Ubiquitin-conjugating enzyme 2C (UBE2C) was found to be one of the key genes in HCC [8]. HCC samples had high expression of UBE2C than adjacent normal biopsies [9].
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