A fine morphological study of germ cells in asymmetrically developing right and left ovaries of the chick

Abstract

Germ cells of the chick ovary which develop asymmetrically on the right and left sides of the embryo were observed by electron microscopy from 7 to 17 days of incubation. Germ cells showed different patterns in the right and left ovaries, and also differences in the cortex and in the medulla. At 7 to 9 days, germ cells were individually present in both the right and left ovaries. They proliferated from 10 days onward and formed many cell aggregates. In each aggregate, germ cells were joined by intercellular bridges, and their cell cycles were synchronized. Although meiotic features of the germ cells were frequently found in the cortex of the left ovaries at 16 days, some germ cells exhibited these features at earlier days. In the meiotic germ cells, the "Balbiani body," which was composed of a concentration of cell organelles shifted to one pole of the cell, was always seen. The appearance of a meiotic nucleus and Balbiani body was characteristic of germ cells located in the left ovarian cortex, whereas those were never seen in the left medulla and in the right ovary. At 16 and 17 days, some germ cells were released into the lacunae of the medulla of both the right and left ovaries. The meaning of this phenomenon is discussed. Degenerating germ cells were frequently observed in the right ovary, but rarely in the left.