Abstract
An easy method for harvesting Mycoplasma pneumoniae ( M.pn.) cells from batch cultures by a tangential flow filtration device is described. The quality of the resulting cell suspension is monitored by two common serological tests; complement fixation test (CFT) and enzyme-linked immunosorbent assay (EIA). For CFT a lipid extract of the M.pn cell suspension is used as antigen and the remaining lipid-depleted cells are the antigen for EIA. No difference is observed between this lipid antigen and the commercial one when the respective CFT results are compared. The ‘protein antigen’ seems to be sensitive for detecting titer rises between paired sera of patients with an enfectious illness. An additional advantage is that the EIA allows detection of antibodies belonging to different immunoglobulin classes.
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